Fluorescence Spectroscopy

With its commitment to technological innovation, Alava Ingenieros offers a wide range of HORIBA products including steady-state fluorescence systems, fluorescence lifetime, hybrid instruments and microscopy-based solutions. This equipment is essential for analysis and scientific research in various applications.

What is Fluorescence Spectroscopy?

Fluorescence spectroscopy is an analytical technique that measures the light emitted by a substance when excited by a specific light source. This method is based on the phenomenon of fluorescence, where absorbing molecules re-emit light upon returning to a lower energy state. Fluorescence spectroscopy stands out for its high sensitivity and specificity, which makes it indispensable in fields such as biochemistry, pharmacology and medical diagnostics.

In contrast to UV-visible spectroscopy, fluorescence spectroscopy offers higher sensitivity and the ability to analyze complex molecular structures. In addition, it allows the detection of very low concentrations of analytes and the precise identification of multiple components in a sample.

Advantages of Fluorescence Spectroscopy

Fluorescence spectroscopy has several key advantages:

– High Sensitivity: Able to detect very low concentrations of analytes.

– Specificity: Allows the identification and quantification of multiple components in a sample thanks to the unique spectral signature of each fluorophore.

– Versatility: It is applied in liquid, solid and gas analysis, adapting to diverse research and quality control environments.

HORIBA Scientific Fluorescence Spectroscopy Systems

HORIBA Scientific offers an advanced range of fluorescence spectroscopy systems designed to meet the needs of various industrial and research applications:

• Duetta™is a 3-in-1 spectrofluorimeter that simultaneously combines the functions of fluorescence and absorbance spectrometers. Thanks to its integrated high-speed CCD detector, the Duetta can acquire a full spectrum from 250 nm to 1100 nm in less than one second, making it the fastest fluorescence spectrometer on the market.
• Aqualog®: Is the only instrument that simultaneously measures absorbance spectra and fluorescence excitation-emission matrices. It offers up to 100 times faster data acquisition speed than other systems.
• FluoroMax+is an easy-to-use and affordable benchtop model. It is a compact spectrofluorimeter, but offers the highest sensitivity in fluorescence research, as well as features not found in most benchtop fluorescence detection systems. Fluoromax+ is the latest high-performance generation that offers a broad spectrum of analysis, up to 1700nm. It also allows time-to-live measurements (TCSPC) with measurement times as short as 25ps.
• QuantaMaster 8000QuantaMaster 8000: is a high-performance, high-sensitivity, modular system for fluorescence studies. It is ideal for advanced research requiring detailed and accurate analysis.
• Deltaflexis a time-correlated single photon counting system designed to measure luminescence lifetime over a wide dynamic range.
• DeltaProDeltaPro: facilitates the use of the time-correlated single photon counting (TCSPC) method in any laboratory, providing a powerful tool for fluorescence dynamics studies.
• Nanologis a spectrofluorometer designed specifically for nanotechnology and nanomaterials research, offering both steady-state and lifetime fluorescence.
• DeltaTimeis a modular system for fluorescence lifetime measurement using TCSPC, ideal for applications requiring high precision analysis.
• Veloci A-TEEM BioPharma contains an innovative column-free molecular fingerprinting technology designed for the biopharmaceutical and pharmaceutical industries. By integrating the selectivity of chromatography with the advantages of optical spectroscopy, Veloci offers a fast, simple and cost-effective method for comprehensive component analysis in a variety of industrial quality control and quality assurance applications in Bioscience.
• FL-QMFL-QM: Represents the culmination of HORIBA’s decades of industry-leading experience in the development and manufacture of spectrofluorimeters with the highest level of performance and versatility.
  
  • The Fluorolog-QM, with its exquisite, optically perfect, fully reflective optics, combined with a multitude of light source and detector options and sample handling accessories, provides the highest sensitivity and versatility of any spectrofluorimeter on the market.